Laser Desorption/ionization Mass Spectrometry Using Tunable Nanoporous Structures
نویسندگان
چکیده
ABSTRACT The performance of porous silicon and cobalt glancing angle deposited (GLAD) thin films for matrix-free laser desorption/ionization-MS analysis of carbohydrates, peptides and drugs with molecular weights < 2,500 Dalton is presented. The performance of these films for selected peptides and small drugs detection is similar to the best reported for desorption/ionization on electrochemically etched porous silicon with detection limits in the femtomole to sub-femtomole range, and was superior to matrix-assisted laser desorption/ionization (MALDI)-MS in terms of background signal and spot-to-spot reproducibility.
منابع مشابه
Laser Chemistry: Nanoporous Carbons Derived from Metal-Organic Frameworks as Novel Matrices for Surface-Assisted Laser Desorption/Ionization Mass Spectrometry (Small 15/2016).
On page 2057, a Lewis acid porous carbon material with tunable hydrophilicity is prepared by temperature controlled pyrolysis of Al based metal-organic frameworks, by C.-H. Lin, H.-Y. Huang, S. Ma, and co-workers. This porous carbon highlights its low heat capacity, and high water uptake, which acts as efficient laser-absorbing matrix/sorbent for detecting trace levels of target compounds.
متن کاملNanoporous Carbons Derived from Metal-Organic Frameworks as Novel Matrices for Surface-Assisted Laser Desorption/Ionization Mass Spectrometry.
Surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) represents a powerful tool for the analysis of biomolecules, synthetic polymers, and even small organic compounds; its performances largely depend on the type of matrix materials utilized. Here, for the first time the employment of nanoporous carbons derived from metal-organic frameworks (MOFs) as novel matrices for SALDI...
متن کاملCompact tunable Cr:LiSAF laser for infrared matrix-assisted laser desorption/ionization.
A tunable Cr:LiSAF laser-pumped optical parametric oscillator was used for mid-infrared matrix-assisted laser desorption/ionization (MALDI) mass spectrometry experiments. The mass spectra of substance P, bovine insulin and pd(T)10 in the 2.88-2.96 microns range showed excellent single shot signal quality (signal-to-noise-ratio, resolution) but poor shot-to-shot reproducibility. The reproducibil...
متن کاملContinuous Flow Atmospheric Pressure Laser Desorption/Ionization Using a 6–7-µm-Band Mid-Infrared Tunable Laser for Biomolecular Mass Spectrometry
A continuous flow atmospheric pressure laser desorption/ionization technique using a porous stainless steel probe and a 6-7-µm-band mid-infrared tunable laser was developed. This ion source is capable of direct ionization from a continuous flow with a high temporal stability. The 6-7-µm wavelength region corresponds to the characteristic absorption bands of various molecular vibration modes, in...
متن کاملAnalysis of the Photo Conversion of Asphaltenes Using Laser Desorption Ionization Mass Spectrometry: Fragmentation, Ring Fusion, and Fullerene Formation
The conversion or photo conversion of asphaltenes to polycyclic aromatic hydrocarbons (PAH’s) promoted by a laser source is analyzed using both experimental and theoretical methods. We propose that during measurements performed at an intermediate laser power, fragmentation to afford PAH’s and ring fusion to yield fused PAH’s (FPAH’s) may occur either within molecular clusters (resin case) or wi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2011